Primer Details for TRINITY_DN15624_c0_g1_i1 | |||
|---|---|---|---|
| (AT)6: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCGTTCCTCTCATTGGCA | AGCTCAATTGAAAACTCCAAACTGT | Start: 84 |
| Tm | 60.035 | 59.872 | End: 304 |
| Size | 20 | 25 | 221 |
| Primer 2 details | |||
| Sequence | GGCCTCCATAAGTATCATCTCATCA | TGCCAATGAGAGGAACGCAT | Start: 0 |
| Tm | 59.811 | 60.035 | End: 103 |
| Size | 25 | 20 | 104 |
| Primer 3 details | |||
| Sequence | ATGCGTTCCTCTCATTGGCA | GCTCAATTGAAAACTCCAAACTGT | Start: 84 |
| Tm | 60.035 | 58.649 | End: 303 |
| Size | 20 | 24 | 220 |
| Primer 4 details | |||
| Sequence | ATGCGTTCCTCTCATTGGCA | AGCTCAATTGAAAACTCCAAACTG | Start: 84 |
| Tm | 60.035 | 58.405 | End: 304 |
| Size | 20 | 24 | 221 |
| Primer 5 details | |||
| Sequence | GGCCTCCATAAGTATCATCTCATC | TGCCAATGAGAGGAACGCAT | Start: 0 |
| Tm | 58.331 | 60.035 | End: 103 |
| Size | 24 | 20 | 104 |
