ssr_primer
Primer Details for TRINITY_DN15350_c0_g1_i1 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGCCGCTCACAACGTC | CCAGACAAGGCTGACACTGT | Start: 380 |
| Tm | 60.248 | 59.893 | End: 514 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | CTTTTTGCCGCTCACAACGT | CCAGACAAGGCTGACACTGT | Start: 379 |
| Tm | 60.248 | 59.893 | End: 514 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | ACAGTGTCAGCCTTGTCTGG | GCTTACGTGACAACCCGTCT | Start: 495 |
| Tm | 59.893 | 60.319 | End: 617 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | ACAGTGTCAGCCTTGTCTGG | AGCTTACGTGACAACCCGTC | Start: 495 |
| Tm | 59.893 | 60.319 | End: 618 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | TCCTTTTTGCCGCTCACAAC | CCAGACAAGGCTGACACTGT | Start: 377 |
| Tm | 59.616 | 59.893 | End: 514 |
| Size | 20 | 20 | 138 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGCCGCTCACAACGTC | CCAGACAAGGCTGACACTGT | Start: 380 |
| Tm | 60.248 | 59.893 | End: 514 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | CTTTTTGCCGCTCACAACGT | CCAGACAAGGCTGACACTGT | Start: 379 |
| Tm | 60.248 | 59.893 | End: 514 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | ACAGTGTCAGCCTTGTCTGG | GCTTACGTGACAACCCGTCT | Start: 495 |
| Tm | 59.893 | 60.319 | End: 617 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | ACAGTGTCAGCCTTGTCTGG | AGCTTACGTGACAACCCGTC | Start: 495 |
| Tm | 59.893 | 60.319 | End: 618 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | TCCTTTTTGCCGCTCACAAC | CCAGACAAGGCTGACACTGT | Start: 377 |
| Tm | 59.616 | 59.893 | End: 514 |
| Size | 20 | 20 | 138 |
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