Primer Details for TRINITY_DN15182_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAGAGATACACAAAACTACCCT | TGCTTGTTGTATTCGTTTACATGC | Start: 76 |
| Tm | 59.214 | 58.849 | End: 175 |
| Size | 25 | 24 | 100 |
| Primer 2 details |
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| Sequence | AGAGATACACAAAACTACCCTTCCA | TGTTTGATGCTTGTTGTATTCGT | Start: 80 |
| Tm | 59.454 | 57.569 | End: 182 |
| Size | 25 | 23 | 103 |
| Primer 3 details |
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| Sequence | AGGAAGAGATACACAAAACTACCCT | TGCTTGTTGTATTCGTTTACATGCA | Start: 76 |
| Tm | 59.214 | 60.278 | End: 175 |
| Size | 25 | 25 | 100 |
| Primer 4 details |
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| Sequence | GAGGAAGAGATACACAAAACTACCCT | GCTTGTTGTATTCGTTTACATGCAC | Start: 75 |
| Tm | 60.071 | 59.941 | End: 174 |
| Size | 26 | 25 | 100 |
| Primer 5 details |
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| Sequence | GAGGAAGAGATACACAAAACTACCC | GCTTGTTGTATTCGTTTACATGCAC | Start: 75 |
| Tm | 58.893 | 59.941 | End: 174 |
| Size | 25 | 25 | 100 |
