ssr_primer
Primer Details for TRINITY_DN15158_c0_g1_i1 |
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| (A)10tccttttctaacaatacatttat(A)10ttaaaatctaactacgtgaaggtattttccataacaaatctactagtaccattttcacatatcagatc(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCACCAGTTGTTATCTTCTGT | ACAAACGATGGAGTGTATGCA | Start: 35 |
| Tm | 60.181 | 57.948 | End: 270 |
| Size | 23 | 21 | 236 |
| Primer 2 details |
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| Sequence | TGCCACCAGTTGTTATCTTCTGT | AGGCCTAACAGAAACAAACGA | Start: 35 |
| Tm | 60.181 | 57.797 | End: 283 |
| Size | 23 | 21 | 249 |
| Primer 3 details |
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| Sequence | TGCCACCAGTTGTTATCTTCTG | ACAAACGATGGAGTGTATGCA | Start: 35 |
| Tm | 58.589 | 57.948 | End: 270 |
| Size | 22 | 21 | 236 |
| Primer 4 details |
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| Sequence | GCCACCAGTTGTTATCTTCTGT | ACAAACGATGGAGTGTATGCA | Start: 36 |
| Tm | 58.588 | 57.948 | End: 270 |
| Size | 22 | 21 | 235 |
| Primer 5 details |
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| Sequence | TGCCACCAGTTGTTATCTTCTGT | GGCCTAACAGAAACAAACGATGG | Start: 35 |
| Tm | 60.181 | 60.366 | End: 282 |
| Size | 23 | 23 | 248 |
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