ssr_primer
Primer Details for TRINITY_DN14833_c0_g1_i1 |
|---|
| (CTT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCAAATCCACTGCCACCC | TTGCGATTTTGGGCAAAGGG | Start: 327 |
| Tm | 59.962 | 59.965 | End: 568 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | CCCTTTGCCCAAAATCGCAA | AACCAAACTCCCATCGCCAT | Start: 549 |
| Tm | 59.965 | 59.96 | End: 744 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | ATGGCGATGGGAGTTTGGTT | AAGTAGCGAGTTGACTGGGC | Start: 725 |
| Tm | 59.96 | 60.037 | End: 910 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | ATGGCGATGGGAGTTTGGTT | CGTGTGGGCACATCCAAAAG | Start: 725 |
| Tm | 59.96 | 60.039 | End: 837 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | GCCCAGTCAACTCGCTACTT | CGAGAATCAGTGGACTCGGG | Start: 891 |
| Tm | 60.037 | 59.899 | End: 1003 |
| Size | 20 | 20 | 113 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCAAATCCACTGCCACCC | TTGCGATTTTGGGCAAAGGG | Start: 327 |
| Tm | 59.962 | 59.965 | End: 568 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | CCCTTTGCCCAAAATCGCAA | AACCAAACTCCCATCGCCAT | Start: 549 |
| Tm | 59.965 | 59.96 | End: 744 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | ATGGCGATGGGAGTTTGGTT | AAGTAGCGAGTTGACTGGGC | Start: 725 |
| Tm | 59.96 | 60.037 | End: 910 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | ATGGCGATGGGAGTTTGGTT | CGTGTGGGCACATCCAAAAG | Start: 725 |
| Tm | 59.96 | 60.039 | End: 837 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | GCCCAGTCAACTCGCTACTT | CGAGAATCAGTGGACTCGGG | Start: 891 |
| Tm | 60.037 | 59.899 | End: 1003 |
| Size | 20 | 20 | 113 |
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