Primer Details for TRINITY_DN14821_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAACACATACTTCTCACCCT | AGTCTAGCTTCATCCACGAGT | Start: 16 |
| Tm | 57.025 | 58.271 | End: 238 |
| Size | 22 | 21 | 223 |
| Primer 2 details | |||
| Sequence | TCTTTTAGCAATGGTGGAAACATGT | AGTCTAGCTTCATCCACGAGT | Start: 87 |
| Tm | 59.696 | 58.271 | End: 238 |
| Size | 25 | 21 | 152 |
| Primer 3 details | |||
| Sequence | TTCAACACATACTTCTCACCCT | AGTCTAGCTTCATCCACGAGTT | Start: 16 |
| Tm | 57.025 | 58.907 | End: 238 |
| Size | 22 | 22 | 223 |
| Primer 4 details | |||
| Sequence | ATTCAACACATACTTCTCACCCT | AGTCTAGCTTCATCCACGAGT | Start: 15 |
| Tm | 57.495 | 58.271 | End: 238 |
| Size | 23 | 21 | 224 |
| Primer 5 details | |||
| Sequence | ACTCGTGGATGAAGCTAGACT | AAAGACGTGATTTGGATGTGTTT | Start: 218 |
| Tm | 58.271 | 57.486 | End: 393 |
| Size | 21 | 23 | 176 |
