ssr_primer
Primer Details for TRINITY_DN14722_c1_g1_i1 |
|---|
| (CGA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGACAAGCCCACGTTATT | TCGTCGACCACTCCATCTCT | Start: 457 |
| Tm | 59.964 | 60.035 | End: 638 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | ACCGACAAGCCCACGTTATT | ATCGCCATCGCCATCATCAT | Start: 457 |
| Tm | 59.964 | 60.036 | End: 657 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | AGAGATGGAGTGGTCGACGA | TCGCCTTTTTCTCCGACCTC | Start: 619 |
| Tm | 60.035 | 60.038 | End: 880 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | AGATCGAAGCCGCTTCACAA | AATAACGTGGGCTTGTCGGT | Start: 280 |
| Tm | 60.037 | 59.964 | End: 476 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | ATGATGATGGCGATGGCGAT | TCGCCTTTTTCTCCGACCTC | Start: 638 |
| Tm | 60.036 | 60.038 | End: 880 |
| Size | 20 | 20 | 243 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGACAAGCCCACGTTATT | TCGTCGACCACTCCATCTCT | Start: 457 |
| Tm | 59.964 | 60.035 | End: 638 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | ACCGACAAGCCCACGTTATT | ATCGCCATCGCCATCATCAT | Start: 457 |
| Tm | 59.964 | 60.036 | End: 657 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | AGAGATGGAGTGGTCGACGA | TCGCCTTTTTCTCCGACCTC | Start: 619 |
| Tm | 60.035 | 60.038 | End: 880 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | AGATCGAAGCCGCTTCACAA | AATAACGTGGGCTTGTCGGT | Start: 280 |
| Tm | 60.037 | 59.964 | End: 476 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | ATGATGATGGCGATGGCGAT | TCGCCTTTTTCTCCGACCTC | Start: 638 |
| Tm | 60.036 | 60.038 | End: 880 |
| Size | 20 | 20 | 243 |
(TC)6tatctttatgtatgtgtgtgtg(TA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGACAAGCCCACGTTATT | TCGTCGACCACTCCATCTCT | Start: 457 |
| Tm | 59.964 | 60.035 | End: 638 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | ACCGACAAGCCCACGTTATT | ATCGCCATCGCCATCATCAT | Start: 457 |
| Tm | 59.964 | 60.036 | End: 657 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | AGAGATGGAGTGGTCGACGA | TCGCCTTTTTCTCCGACCTC | Start: 619 |
| Tm | 60.035 | 60.038 | End: 880 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | AGATCGAAGCCGCTTCACAA | AATAACGTGGGCTTGTCGGT | Start: 280 |
| Tm | 60.037 | 59.964 | End: 476 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | ATGATGATGGCGATGGCGAT | TCGCCTTTTTCTCCGACCTC | Start: 638 |
| Tm | 60.036 | 60.038 | End: 880 |
| Size | 20 | 20 | 243 |
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