ssr_primer
Primer Details for TRINITY_DN14714_c2_g2_i9 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGCGGGTCTGCTAGGACTT | GCGCCAAAAGATCCCGATTC | Start: 4608 |
| Tm | 60.033 | 59.971 | End: 4757 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | CGTTACAGAGGGCCTCGTTT | GCTTTTCCGGCGATACGAAC | Start: 452 |
| Tm | 60.038 | 59.974 | End: 669 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | CTCTTTCTTGGGCGGGACAT | GCGCCAAAAGATCCCGATTC | Start: 4632 |
| Tm | 60.035 | 59.971 | End: 4757 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | TCTTTCTTGGGCGGGACATC | GCGCCAAAAGATCCCGATTC | Start: 4633 |
| Tm | 60.035 | 59.971 | End: 4757 |
| Size | 20 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | AAAAACCAATCGCGTTCCGG | GCTTTTCCGGCGATACGAAC | Start: 542 |
| Tm | 60.04 | 59.974 | End: 669 |
| Size | 20 | 20 | 128 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGCGGGTCTGCTAGGACTT | GCGCCAAAAGATCCCGATTC | Start: 4608 |
| Tm | 60.033 | 59.971 | End: 4757 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | CGTTACAGAGGGCCTCGTTT | GCTTTTCCGGCGATACGAAC | Start: 452 |
| Tm | 60.038 | 59.974 | End: 669 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | CTCTTTCTTGGGCGGGACAT | GCGCCAAAAGATCCCGATTC | Start: 4632 |
| Tm | 60.035 | 59.971 | End: 4757 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | TCTTTCTTGGGCGGGACATC | GCGCCAAAAGATCCCGATTC | Start: 4633 |
| Tm | 60.035 | 59.971 | End: 4757 |
| Size | 20 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | AAAAACCAATCGCGTTCCGG | GCTTTTCCGGCGATACGAAC | Start: 542 |
| Tm | 60.04 | 59.974 | End: 669 |
| Size | 20 | 20 | 128 |
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