ssr_primer
Primer Details for TRINITY_DN14711_c5_g1_i3 |
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| (T)30gtcttctttaagattgtag(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGCAGAGGCCTTTGTCTT | GCAACCGTATGCAGAAGCTG | Start: 3596 |
| Tm | 59.963 | 59.902 | End: 3763 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | ACTTACACCCAAACGGCACA | AGGCCTGTCTAATGGAACGC | Start: 1593 |
| Tm | 60.107 | 60.108 | End: 1755 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
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| Sequence | TACACCCAAACGGCACAAGA | AGGCCTGTCTAATGGAACGC | Start: 1596 |
| Tm | 59.819 | 60.108 | End: 1755 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | TCACTTACACCCAAACGGCA | AGGCCTGTCTAATGGAACGC | Start: 1591 |
| Tm | 59.819 | 60.108 | End: 1755 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
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| Sequence | GCATGCAAAGCGTTTTGAGC | TCAGCAAACAAAGTCCGGGA | Start: 2420 |
| Tm | 59.838 | 59.818 | End: 2549 |
| Size | 20 | 20 | 130 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGCAGAGGCCTTTGTCTT | GCAACCGTATGCAGAAGCTG | Start: 3596 |
| Tm | 59.963 | 59.902 | End: 3763 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | ACTTACACCCAAACGGCACA | AGGCCTGTCTAATGGAACGC | Start: 1593 |
| Tm | 60.107 | 60.108 | End: 1755 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | TACACCCAAACGGCACAAGA | AGGCCTGTCTAATGGAACGC | Start: 1596 |
| Tm | 59.819 | 60.108 | End: 1755 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | TCACTTACACCCAAACGGCA | AGGCCTGTCTAATGGAACGC | Start: 1591 |
| Tm | 59.819 | 60.108 | End: 1755 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GCATGCAAAGCGTTTTGAGC | TCAGCAAACAAAGTCCGGGA | Start: 2420 |
| Tm | 59.838 | 59.818 | End: 2549 |
| Size | 20 | 20 | 130 |
(TC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGCAGAGGCCTTTGTCTT | GCAACCGTATGCAGAAGCTG | Start: 3596 |
| Tm | 59.963 | 59.902 | End: 3763 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | ACTTACACCCAAACGGCACA | AGGCCTGTCTAATGGAACGC | Start: 1593 |
| Tm | 60.107 | 60.108 | End: 1755 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | TACACCCAAACGGCACAAGA | AGGCCTGTCTAATGGAACGC | Start: 1596 |
| Tm | 59.819 | 60.108 | End: 1755 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | TCACTTACACCCAAACGGCA | AGGCCTGTCTAATGGAACGC | Start: 1591 |
| Tm | 59.819 | 60.108 | End: 1755 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GCATGCAAAGCGTTTTGAGC | TCAGCAAACAAAGTCCGGGA | Start: 2420 |
| Tm | 59.838 | 59.818 | End: 2549 |
| Size | 20 | 20 | 130 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGCAGAGGCCTTTGTCTT | GCAACCGTATGCAGAAGCTG | Start: 3596 |
| Tm | 59.963 | 59.902 | End: 3763 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
|---|
| Sequence | ACTTACACCCAAACGGCACA | AGGCCTGTCTAATGGAACGC | Start: 1593 |
| Tm | 60.107 | 60.108 | End: 1755 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | TACACCCAAACGGCACAAGA | AGGCCTGTCTAATGGAACGC | Start: 1596 |
| Tm | 59.819 | 60.108 | End: 1755 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | TCACTTACACCCAAACGGCA | AGGCCTGTCTAATGGAACGC | Start: 1591 |
| Tm | 59.819 | 60.108 | End: 1755 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GCATGCAAAGCGTTTTGAGC | TCAGCAAACAAAGTCCGGGA | Start: 2420 |
| Tm | 59.838 | 59.818 | End: 2549 |
| Size | 20 | 20 | 130 |
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