ssr_primer
Primer Details for TRINITY_DN14705_c3_g2_i1 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCGACGCCAGGATGATAA | GAACCTTTCCTCTCCCCAGC | Start: 46 |
| Tm | 59.968 | 60.035 | End: 271 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | TACTGTAGGGGAGGTCCTGC | GAACCTTTCCTCTCCCCAGC | Start: 17 |
| Tm | 60.032 | 60.035 | End: 271 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | AATAGCTCGACGCCAGGATG | GAACCTTTCCTCTCCCCAGC | Start: 42 |
| Tm | 59.967 | 60.035 | End: 271 |
| Size | 20 | 20 | 230 |
| Primer 4 details |
|---|
| Sequence | GCTGGGGAGAGGAAAGGTTC | CTTCCCTTTTTGGCCGGTTG | Start: 252 |
| Tm | 60.035 | 59.967 | End: 410 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GCTCGACGCCAGGATGATAA | GCCATTTGCCATTTCGGGTT | Start: 46 |
| Tm | 59.968 | 60.036 | End: 253 |
| Size | 20 | 20 | 208 |
(T)10atcaaagcaattagtggaattttcctaatgttttgagcagataaatatgaaattccttttcctttttgtaggctatgt(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCGACGCCAGGATGATAA | GAACCTTTCCTCTCCCCAGC | Start: 46 |
| Tm | 59.968 | 60.035 | End: 271 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | TACTGTAGGGGAGGTCCTGC | GAACCTTTCCTCTCCCCAGC | Start: 17 |
| Tm | 60.032 | 60.035 | End: 271 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | AATAGCTCGACGCCAGGATG | GAACCTTTCCTCTCCCCAGC | Start: 42 |
| Tm | 59.967 | 60.035 | End: 271 |
| Size | 20 | 20 | 230 |
| Primer 4 details |
|---|
| Sequence | GCTGGGGAGAGGAAAGGTTC | CTTCCCTTTTTGGCCGGTTG | Start: 252 |
| Tm | 60.035 | 59.967 | End: 410 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GCTCGACGCCAGGATGATAA | GCCATTTGCCATTTCGGGTT | Start: 46 |
| Tm | 59.968 | 60.036 | End: 253 |
| Size | 20 | 20 | 208 |
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