ssr_primer
Primer Details for TRINITY_DN14690_c2_g1_i4 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACAACGGTTGAAGGTTTGG | TCAAATGCAAAACACACGAGT | Start: 159 |
| Tm | 60.268 | 57.226 | End: 288 |
| Size | 21 | 21 | 130 |
| Primer 2 details |
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| Sequence | CGTCTTTGAAACGACAACGGT | TCAAATGCAAAACACACGAGT | Start: 148 |
| Tm | 59.675 | 57.226 | End: 288 |
| Size | 21 | 21 | 141 |
| Primer 3 details |
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| Sequence | ACCGTCTTTGAAACGACAACG | TCAAATGCAAAACACACGAGT | Start: 146 |
| Tm | 59.675 | 57.226 | End: 288 |
| Size | 21 | 21 | 143 |
| Primer 4 details |
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| Sequence | CCGTCTTTGAAACGACAACGG | TCAAATGCAAAACACACGAGT | Start: 147 |
| Tm | 60.333 | 57.226 | End: 288 |
| Size | 21 | 21 | 142 |
| Primer 5 details |
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| Sequence | CGACAACGGTTGAAGGTTTGG | TGACACTCTGAAAATGAGAGGGA | Start: 159 |
| Tm | 60.268 | 59.096 | End: 418 |
| Size | 21 | 23 | 260 |
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