Primer Details for TRINITY_DN1468_c0_g1_i1 | |||
|---|---|---|---|
| (T)17: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAGAACAGCTAAGACTAGTTCA | TGGCTCAAGAAAGTCCGGAG | Start: 155 |
| Tm | 58.922 | 59.678 | End: 266 |
| Size | 24 | 20 | 112 |
| Primer 2 details | |||
| Sequence | ACAGCTAAGACTAGTTCATCATGCT | TGGCTCAAGAAAGTCCGGAG | Start: 161 |
| Tm | 59.872 | 59.678 | End: 266 |
| Size | 25 | 20 | 106 |
| Primer 3 details | |||
| Sequence | ACAGCTAAGACTAGTTCATCATGC | TGGCTCAAGAAAGTCCGGAG | Start: 161 |
| Tm | 58.644 | 59.678 | End: 266 |
| Size | 24 | 20 | 106 |
| Primer 4 details | |||
| Sequence | TGGAGAACAGCTAAGACTAGTTCA | GGCTCAAGAAAGTCCGGAGA | Start: 155 |
| Tm | 58.922 | 59.391 | End: 265 |
| Size | 24 | 20 | 111 |
| Primer 5 details | |||
| Sequence | TGGAGAACAGCTAAGACTAGTTCA | AGTCCGGAGACAGGAGATGA | Start: 155 |
| Tm | 58.922 | 59.374 | End: 255 |
| Size | 24 | 20 | 101 |
