ssr_primer
Primer Details for TRINITY_DN14663_c5_g4_i1 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACACAACGGCCTTGGAAAG | TAAGCGATGGCCAAGCATCA | Start: 861 |
| Tm | 59.969 | 60.107 | End: 1043 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | CACACAACGGCCTTGGAAAG | TTGGCCGCCTATGTGAGAAG | Start: 861 |
| Tm | 59.969 | 60.108 | End: 982 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | CACACAACGGCCTTGGAAAG | AAGTGCGATGGGAAGTGTGT | Start: 861 |
| Tm | 59.969 | 59.891 | End: 965 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | TCCGCCCAAGGGAATCAATC | AGAACAGTTGTTGGGGCCAA | Start: 411 |
| Tm | 60.107 | 60.033 | End: 587 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
|---|
| Sequence | TCGAACAAGTGTACAGGGGC | TAAGCGATGGCCAAGCATCA | Start: 906 |
| Tm | 59.966 | 60.107 | End: 1043 |
| Size | 20 | 20 | 138 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACACAACGGCCTTGGAAAG | TAAGCGATGGCCAAGCATCA | Start: 861 |
| Tm | 59.969 | 60.107 | End: 1043 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | CACACAACGGCCTTGGAAAG | TTGGCCGCCTATGTGAGAAG | Start: 861 |
| Tm | 59.969 | 60.108 | End: 982 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | CACACAACGGCCTTGGAAAG | AAGTGCGATGGGAAGTGTGT | Start: 861 |
| Tm | 59.969 | 59.891 | End: 965 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | TCCGCCCAAGGGAATCAATC | AGAACAGTTGTTGGGGCCAA | Start: 411 |
| Tm | 60.107 | 60.033 | End: 587 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
|---|
| Sequence | TCGAACAAGTGTACAGGGGC | TAAGCGATGGCCAAGCATCA | Start: 906 |
| Tm | 59.966 | 60.107 | End: 1043 |
| Size | 20 | 20 | 138 |
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