ssr_primer
Primer Details for TRINITY_DN14651_c1_g6_i1 |
|---|
| (CT)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTTCGGAAATGAAGGCGA | CGGCTTGTTCGCTGTATTCG | Start: 510 |
| Tm | 60.036 | 59.975 | End: 623 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | CGAATACAGCGAACAAGCCG | TTCCGCTCGACATTGCTCTT | Start: 604 |
| Tm | 59.975 | 60.037 | End: 748 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | CGAATACAGCGAACAAGCCG | TTCTTCCGCTCGACATTGCT | Start: 604 |
| Tm | 59.975 | 60.037 | End: 751 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
|---|
| Sequence | TTGCTTCTTCATCCGCGAGT | CGGCTTGTTCGCTGTATTCG | Start: 481 |
| Tm | 60.037 | 59.975 | End: 623 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
|---|
| Sequence | ATGGCGGATTCAGTGAAGCA | TTCCGCTCGACATTGCTCTT | Start: 552 |
| Tm | 60.035 | 60.037 | End: 748 |
| Size | 20 | 20 | 197 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTTCGGAAATGAAGGCGA | CGGCTTGTTCGCTGTATTCG | Start: 510 |
| Tm | 60.036 | 59.975 | End: 623 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | CGAATACAGCGAACAAGCCG | TTCCGCTCGACATTGCTCTT | Start: 604 |
| Tm | 59.975 | 60.037 | End: 748 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | CGAATACAGCGAACAAGCCG | TTCTTCCGCTCGACATTGCT | Start: 604 |
| Tm | 59.975 | 60.037 | End: 751 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
|---|
| Sequence | TTGCTTCTTCATCCGCGAGT | CGGCTTGTTCGCTGTATTCG | Start: 481 |
| Tm | 60.037 | 59.975 | End: 623 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
|---|
| Sequence | ATGGCGGATTCAGTGAAGCA | TTCCGCTCGACATTGCTCTT | Start: 552 |
| Tm | 60.035 | 60.037 | End: 748 |
| Size | 20 | 20 | 197 |
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