Primer Details for TRINITY_DN14620_c1_g1_i1 |
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| (A)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAATTTGATTAGAAATGAGTGGAGA | CAAATGCTCGTTGGTGGACG | Start: 39 |
| Tm | 59.11 | 60.11 | End: 139 |
| Size | 27 | 20 | 101 |
| Primer 2 details |
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| Sequence | ACCAATTTGATTAGAAATGAGTGGAG | CAAATGCTCGTTGGTGGACG | Start: 39 |
| Tm | 57.94 | 60.11 | End: 139 |
| Size | 26 | 20 | 101 |
| Primer 3 details |
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| Sequence | TGCAATTTCTCTCGTCCACCA | ACGACTTGTTTTATAAGCTAAACACGA | Start: 108 |
| Tm | 59.928 | 59.83 | End: 209 |
| Size | 21 | 27 | 102 |
| Primer 4 details |
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| Sequence | TGCAATTTCTCTCGTCCACCA | ACGACTTGTTTTATAAGCTAAACACG | Start: 108 |
| Tm | 59.928 | 58.714 | End: 209 |
| Size | 21 | 26 | 102 |
| Primer 5 details |
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| Sequence | CCAATTTGATTAGAAATGAGTGGAGA | CAAATGCTCGTTGGTGGACG | Start: 40 |
| Tm | 57.715 | 60.11 | End: 139 |
| Size | 26 | 20 | 100 |
