ssr_primer
Primer Details for TRINITY_DN14618_c2_g1_i5 |
|---|
| (AG)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAGCCAATTCCGCTCGAT | CGTAATGCTTCGGCGTTCAG | Start: 3583 |
| Tm | 59.893 | 59.975 | End: 3772 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | ATCGAGCGGAATTGGCTGAT | Start: 3441 |
| Tm | 60.034 | 59.893 | End: 3602 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | ATGGCTCGATTTGGAGGGTG | AGTGACACGTCGACTTGCTT | Start: 1662 |
| Tm | 60.107 | 59.898 | End: 1784 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | GCAAGCAGTAGGTCACCACA | CGTAATGCTTCGGCGTTCAG | Start: 3667 |
| Tm | 60.25 | 59.975 | End: 3772 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | TGTGGTGACCTACTGCTTGC | Start: 3441 |
| Tm | 60.034 | 60.25 | End: 3686 |
| Size | 20 | 20 | 246 |
(GA)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAGCCAATTCCGCTCGAT | CGTAATGCTTCGGCGTTCAG | Start: 3583 |
| Tm | 59.893 | 59.975 | End: 3772 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | ATCGAGCGGAATTGGCTGAT | Start: 3441 |
| Tm | 60.034 | 59.893 | End: 3602 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | ATGGCTCGATTTGGAGGGTG | AGTGACACGTCGACTTGCTT | Start: 1662 |
| Tm | 60.107 | 59.898 | End: 1784 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | GCAAGCAGTAGGTCACCACA | CGTAATGCTTCGGCGTTCAG | Start: 3667 |
| Tm | 60.25 | 59.975 | End: 3772 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | TGTGGTGACCTACTGCTTGC | Start: 3441 |
| Tm | 60.034 | 60.25 | End: 3686 |
| Size | 20 | 20 | 246 |
(TG)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAGCCAATTCCGCTCGAT | CGTAATGCTTCGGCGTTCAG | Start: 3583 |
| Tm | 59.893 | 59.975 | End: 3772 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | ATCGAGCGGAATTGGCTGAT | Start: 3441 |
| Tm | 60.034 | 59.893 | End: 3602 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | ATGGCTCGATTTGGAGGGTG | AGTGACACGTCGACTTGCTT | Start: 1662 |
| Tm | 60.107 | 59.898 | End: 1784 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | GCAAGCAGTAGGTCACCACA | CGTAATGCTTCGGCGTTCAG | Start: 3667 |
| Tm | 60.25 | 59.975 | End: 3772 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | TGTGGTGACCTACTGCTTGC | Start: 3441 |
| Tm | 60.034 | 60.25 | End: 3686 |
| Size | 20 | 20 | 246 |
(TG)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAGCCAATTCCGCTCGAT | CGTAATGCTTCGGCGTTCAG | Start: 3583 |
| Tm | 59.893 | 59.975 | End: 3772 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | ATCGAGCGGAATTGGCTGAT | Start: 3441 |
| Tm | 60.034 | 59.893 | End: 3602 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | ATGGCTCGATTTGGAGGGTG | AGTGACACGTCGACTTGCTT | Start: 1662 |
| Tm | 60.107 | 59.898 | End: 1784 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | GCAAGCAGTAGGTCACCACA | CGTAATGCTTCGGCGTTCAG | Start: 3667 |
| Tm | 60.25 | 59.975 | End: 3772 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | TGTGGTGACCTACTGCTTGC | Start: 3441 |
| Tm | 60.034 | 60.25 | End: 3686 |
| Size | 20 | 20 | 246 |
(T)18gccttcctttaactaaatagattcagtaggtgctaattcagtcattataacacacgttattaagggc(A)26: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAGCCAATTCCGCTCGAT | CGTAATGCTTCGGCGTTCAG | Start: 3583 |
| Tm | 59.893 | 59.975 | End: 3772 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | ATCGAGCGGAATTGGCTGAT | Start: 3441 |
| Tm | 60.034 | 59.893 | End: 3602 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | ATGGCTCGATTTGGAGGGTG | AGTGACACGTCGACTTGCTT | Start: 1662 |
| Tm | 60.107 | 59.898 | End: 1784 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | GCAAGCAGTAGGTCACCACA | CGTAATGCTTCGGCGTTCAG | Start: 3667 |
| Tm | 60.25 | 59.975 | End: 3772 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | AATGCCCAATTAGCCCGTCA | TGTGGTGACCTACTGCTTGC | Start: 3441 |
| Tm | 60.034 | 60.25 | End: 3686 |
| Size | 20 | 20 | 246 |
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