ssr_primer
Primer Details for TRINITY_DN14614_c2_g6_i3 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTATCCCCTTGCCCCTTCA | CGGGATGTAACCCCTATGCC | Start: 163 |
| Tm | 60.254 | 59.964 | End: 421 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | GCCTACGGATCAAGCGACAT | TTCAACCAGGACCAGAAGGC | Start: 964 |
| Tm | 60.249 | 59.89 | End: 1197 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | ACCGCTTCTCCGAATAGCTC | ACACCATTTGCCGCTCAAAC | Start: 440 |
| Tm | 59.614 | 59.968 | End: 577 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | ACCGCTTCTCCGAATAGCTC | AGATGTACGGGGTCGGGTAA | Start: 440 |
| Tm | 59.614 | 60.033 | End: 647 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | CCGATTCCCTGTAGTCGAGC | AAAACGGATCAGTCGACCCG | Start: 1536 |
| Tm | 59.969 | 60.389 | End: 1676 |
| Size | 20 | 20 | 141 |
(A)11gaaatattgtttttc(A)22tctacaattg(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTATCCCCTTGCCCCTTCA | CGGGATGTAACCCCTATGCC | Start: 163 |
| Tm | 60.254 | 59.964 | End: 421 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | GCCTACGGATCAAGCGACAT | TTCAACCAGGACCAGAAGGC | Start: 964 |
| Tm | 60.249 | 59.89 | End: 1197 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | ACCGCTTCTCCGAATAGCTC | ACACCATTTGCCGCTCAAAC | Start: 440 |
| Tm | 59.614 | 59.968 | End: 577 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | ACCGCTTCTCCGAATAGCTC | AGATGTACGGGGTCGGGTAA | Start: 440 |
| Tm | 59.614 | 60.033 | End: 647 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | CCGATTCCCTGTAGTCGAGC | AAAACGGATCAGTCGACCCG | Start: 1536 |
| Tm | 59.969 | 60.389 | End: 1676 |
| Size | 20 | 20 | 141 |
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