ssr_primer
Primer Details for TRINITY_DN14606_c1_g3_i20 |
|---|
| (AGT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATCCGCGACTGAGAGAAG | GTCGCGGATCCAATCTCCAT | Start: 1559 |
| Tm | 59.97 | 59.966 | End: 1665 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | ACCGGATGCACTGTGCTTTA | TCCGCTGGATTATCAGACGC | Start: 854 |
| Tm | 59.964 | 59.968 | End: 1090 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
|---|
| Sequence | ACCGGATGCACTGTGCTTTA | CCGCTGGATTATCAGACGCT | Start: 854 |
| Tm | 59.964 | 59.967 | End: 1089 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
|---|
| Sequence | AGCGTCTGATAATCCAGCGG | ATTCCCTCACTCTCACCGGA | Start: 1070 |
| Tm | 59.967 | 59.959 | End: 1169 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | GGATCCGCGACTGAGAGAAG | TCGCGGATCCAATCTCCATC | Start: 1559 |
| Tm | 59.97 | 59.682 | End: 1664 |
| Size | 20 | 20 | 106 |
(AAG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATCCGCGACTGAGAGAAG | GTCGCGGATCCAATCTCCAT | Start: 1559 |
| Tm | 59.97 | 59.966 | End: 1665 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | ACCGGATGCACTGTGCTTTA | TCCGCTGGATTATCAGACGC | Start: 854 |
| Tm | 59.964 | 59.968 | End: 1090 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
|---|
| Sequence | ACCGGATGCACTGTGCTTTA | CCGCTGGATTATCAGACGCT | Start: 854 |
| Tm | 59.964 | 59.967 | End: 1089 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
|---|
| Sequence | AGCGTCTGATAATCCAGCGG | ATTCCCTCACTCTCACCGGA | Start: 1070 |
| Tm | 59.967 | 59.959 | End: 1169 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | GGATCCGCGACTGAGAGAAG | TCGCGGATCCAATCTCCATC | Start: 1559 |
| Tm | 59.97 | 59.682 | End: 1664 |
| Size | 20 | 20 | 106 |
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