ssr_primer
Primer Details for TRINITY_DN14581_c5_g1_i28 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGTGGCCTTCCAAAGTTGC | CGCGCTGATTAGACACGTTG | Start: 2472 |
| Tm | 59.967 | 59.976 | End: 2590 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | CCATTCGCTCAATTGCTCGG | CCTCGACTGAAAGCCTGGTT | Start: 7301 |
| Tm | 59.971 | 59.965 | End: 7436 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | CCATTCGCTCAATTGCTCGG | GGGTCATGCCACTCGGTAAA | Start: 7301 |
| Tm | 59.971 | 60.036 | End: 7508 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | ACATGTTCCTCTCCCGGAGA | AAAAAGGGGCCACTGAACCA | Start: 4086 |
| Tm | 59.959 | 60.032 | End: 4299 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | TTTACCGAGTGGCATGACCC | CCGACTTTGAAAACTGCCCG | Start: 7489 |
| Tm | 60.036 | 60.04 | End: 7766 |
| Size | 20 | 20 | 278 |
(ATAC)5(AT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGTGGCCTTCCAAAGTTGC | CGCGCTGATTAGACACGTTG | Start: 2472 |
| Tm | 59.967 | 59.976 | End: 2590 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | CCATTCGCTCAATTGCTCGG | CCTCGACTGAAAGCCTGGTT | Start: 7301 |
| Tm | 59.971 | 59.965 | End: 7436 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | CCATTCGCTCAATTGCTCGG | GGGTCATGCCACTCGGTAAA | Start: 7301 |
| Tm | 59.971 | 60.036 | End: 7508 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | ACATGTTCCTCTCCCGGAGA | AAAAAGGGGCCACTGAACCA | Start: 4086 |
| Tm | 59.959 | 60.032 | End: 4299 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | TTTACCGAGTGGCATGACCC | CCGACTTTGAAAACTGCCCG | Start: 7489 |
| Tm | 60.036 | 60.04 | End: 7766 |
| Size | 20 | 20 | 278 |
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