Primer Details for TRINITY_DN14578_c2_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAAGGTAACAAGGGTGGC | ACAAACCATCATTTCATGTATGCAGT | Start: 23 |
| Tm | 59.683 | 60.071 | End: 188 |
| Size | 20 | 26 | 166 |
| Primer 2 details |
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| Sequence | GGGGCAGGAAGGAAGAATGT | ACAAACCATCATTTCATGTATGCAGT | Start: 53 |
| Tm | 59.667 | 60.071 | End: 188 |
| Size | 20 | 26 | 136 |
| Primer 3 details |
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| Sequence | AGGTAACAAGGGTGGCAGAC | ACAAACCATCATTTCATGTATGCAGT | Start: 27 |
| Tm | 59.599 | 60.071 | End: 188 |
| Size | 20 | 26 | 162 |
| Primer 4 details |
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| Sequence | GCAAAGGTAACAAGGGTGGC | ACAAACCATCATTTCATGTATGCA | Start: 23 |
| Tm | 59.683 | 57.716 | End: 188 |
| Size | 20 | 24 | 166 |
| Primer 5 details |
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| Sequence | GGGGCAGGAAGGAAGAATGT | ACAAACCATCATTTCATGTATGCA | Start: 53 |
| Tm | 59.667 | 57.716 | End: 188 |
| Size | 20 | 24 | 136 |
