Primer Details for TRINITY_DN14561_c4_g5_i1 |
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| (A)10gaggaataaggaagt(A)10gg(A)10ggaggaataaggaaggaaaaaaagaaaaaaattaaataggaggaataagaaagggaaaaaag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAAAGGAGGAATAAGGAAGGAAAAA | TGGGCAAAAGCTTATTTTTACAATCT | Start: 105 |
| Tm | 57.438 | 58.551 | End: 243 |
| Size | 26 | 26 | 139 |
| Primer 2 details |
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| Sequence | AAAAAGGAGGAATAAGGAAGGAAAAA | TGGGCAAAAGCTTATTTTTACAATC | Start: 105 |
| Tm | 57.438 | 57.334 | End: 243 |
| Size | 26 | 25 | 139 |
| Primer 3 details |
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| Sequence | AAAAAGGAGGAATAAGGAAGGAAAAA | GGGCAAAAGCTTATTTTTACAATCT | Start: 105 |
| Tm | 57.438 | 57.094 | End: 242 |
| Size | 26 | 25 | 138 |
| Primer 4 details |
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| Sequence | AAAAAGGAGGAATAAGGAAGGAAAAA | ATGGGCAAAAGCTTATTTTTACAATCT | Start: 105 |
| Tm | 57.438 | 58.897 | End: 244 |
| Size | 26 | 27 | 140 |
| Primer 5 details |
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| Sequence | AAAAAGGAGGAATAAGGAAGGAAAAA | ATGGGCAAAAGCTTATTTTTACAATC | Start: 105 |
| Tm | 57.438 | 57.732 | End: 244 |
| Size | 26 | 26 | 140 |
