Primer Details for TRINITY_DN14506_c13_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTTTCGCCTTGGTCACCCA | TGCTTTGATACCTTGCATAAAAAGGA | Start: 49 |
| Tm | 60.179 | 59.79 | End: 284 |
| Size | 20 | 26 | 236 |
| Primer 2 details |
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| Sequence | CATTTTCGCCTTGGTCACCC | TGCTTTGATACCTTGCATAAAAAGGA | Start: 48 |
| Tm | 59.756 | 59.79 | End: 284 |
| Size | 20 | 26 | 237 |
| Primer 3 details |
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| Sequence | GCATTTTCGCCTTGGTCACC | TGCTTTGATACCTTGCATAAAAAGGA | Start: 47 |
| Tm | 60.389 | 59.79 | End: 284 |
| Size | 20 | 26 | 238 |
| Primer 4 details |
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| Sequence | ATTTTCGCCTTGGTCACCCA | ACCTTGCATAAAAAGGAGTTTTCTCT | Start: 49 |
| Tm | 60.179 | 59.449 | End: 275 |
| Size | 20 | 26 | 227 |
| Primer 5 details |
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| Sequence | CATTTTCGCCTTGGTCACCC | ACCTTGCATAAAAAGGAGTTTTCTCT | Start: 48 |
| Tm | 59.756 | 59.449 | End: 275 |
| Size | 20 | 26 | 228 |
