ssr_primer
Primer Details for TRINITY_DN14500_c1_g8_i2 |
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| (GATTGG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATGGCACCCCTTTCAAAGC | CAACTCCAGCCTCAATCCGT | Start: 553 |
| Tm | 60.037 | 60.036 | End: 776 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
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| Sequence | CATGGCACCCCTTTCAAAGC | CAACCCCAATCCCAATCCCA | Start: 553 |
| Tm | 60.037 | 59.958 | End: 737 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
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| Sequence | TCCGAAACCAAGTCGCTCAA | CTTGTGCACGGTTCCACTTG | Start: 2029 |
| Tm | 59.895 | 59.971 | End: 2131 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
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| Sequence | ATGGTCACCGCCCATTTCAT | TTGTATGCTGTGGCTCGGAG | Start: 2281 |
| Tm | 60.033 | 60.108 | End: 2427 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
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| Sequence | CCCACTCAAGAAGGCACCAT | TGTTCGAGTTGGGTCCACTG | Start: 1479 |
| Tm | 59.961 | 59.894 | End: 1608 |
| Size | 20 | 20 | 130 |
(CTT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATGGCACCCCTTTCAAAGC | CAACTCCAGCCTCAATCCGT | Start: 553 |
| Tm | 60.037 | 60.036 | End: 776 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
|---|
| Sequence | CATGGCACCCCTTTCAAAGC | CAACCCCAATCCCAATCCCA | Start: 553 |
| Tm | 60.037 | 59.958 | End: 737 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | TCCGAAACCAAGTCGCTCAA | CTTGTGCACGGTTCCACTTG | Start: 2029 |
| Tm | 59.895 | 59.971 | End: 2131 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
|---|
| Sequence | ATGGTCACCGCCCATTTCAT | TTGTATGCTGTGGCTCGGAG | Start: 2281 |
| Tm | 60.033 | 60.108 | End: 2427 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
|---|
| Sequence | CCCACTCAAGAAGGCACCAT | TGTTCGAGTTGGGTCCACTG | Start: 1479 |
| Tm | 59.961 | 59.894 | End: 1608 |
| Size | 20 | 20 | 130 |
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