ssr_primer
Primer Details for TRINITY_DN14483_c3_g1_i30 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCAAGGCTTCCAGTACCA | TGTTCTTGCGCTAGTTCGGT | Start: 1718 |
| Tm | 59.963 | 59.967 | End: 1952 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | TTTACCACTGCCAGTCCGAC | AAGCTAACCGTCCTCCAAGC | Start: 206 |
| Tm | 59.966 | 60.036 | End: 369 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | AGAACAAGGGGCGTATTGGG | GGCACTCTCTTACGGCCTTT | Start: 276 |
| Tm | 60.034 | 60.036 | End: 509 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | GCTTGGAGGACGGTTAGCTT | GGCACTCTCTTACGGCCTTT | Start: 350 |
| Tm | 60.036 | 60.036 | End: 509 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | CTGCAATGGACAATAGGCGC | TCACACTGCTTTGCCTTTGC | Start: 4267 |
| Tm | 59.97 | 59.897 | End: 4400 |
| Size | 20 | 20 | 134 |
(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCAAGGCTTCCAGTACCA | TGTTCTTGCGCTAGTTCGGT | Start: 1718 |
| Tm | 59.963 | 59.967 | End: 1952 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | TTTACCACTGCCAGTCCGAC | AAGCTAACCGTCCTCCAAGC | Start: 206 |
| Tm | 59.966 | 60.036 | End: 369 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | AGAACAAGGGGCGTATTGGG | GGCACTCTCTTACGGCCTTT | Start: 276 |
| Tm | 60.034 | 60.036 | End: 509 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | GCTTGGAGGACGGTTAGCTT | GGCACTCTCTTACGGCCTTT | Start: 350 |
| Tm | 60.036 | 60.036 | End: 509 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | CTGCAATGGACAATAGGCGC | TCACACTGCTTTGCCTTTGC | Start: 4267 |
| Tm | 59.97 | 59.897 | End: 4400 |
| Size | 20 | 20 | 134 |
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