ssr_primer
Primer Details for TRINITY_DN14476_c0_g1_i9 |
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| (A)15gg(A)10gaagagggtttaggtgaagaaggcttagggcattaattttagaaac(ATA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGTGAAAGAAAAGCGCCG | GTGTTATGTTGGCAAGCGCA | Start: 1619 |
| Tm | 59.97 | 60.04 | End: 1743 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
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| Sequence | TTGGTGAAAGAAAAGCGCCG | TGTGTTATGTTGGCAAGCGC | Start: 1619 |
| Tm | 59.97 | 60.04 | End: 1744 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
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| Sequence | TGGCCCATATTCAACCCCAC | ATTTGAGGTGGCTCCAAGGG | Start: 227 |
| Tm | 60.033 | 59.96 | End: 395 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
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| Sequence | TGGCCCATATTCAACCCCAC | CCAAAGGGCTGGAAGGTGAT | Start: 227 |
| Tm | 60.033 | 59.96 | End: 484 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
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| Sequence | ATCACCTTCCAGCCCTTTGG | CAAGACACCACCGCTACTGT | Start: 465 |
| Tm | 59.96 | 59.966 | End: 742 |
| Size | 20 | 20 | 278 |
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