Primer Details for TRINITY_DN14472_c1_g1_i1 |
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| (TTA)8(T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAACAAAGGTTCGTCTGTCCT | GGTTGGTACCGAATGCATATAAGTG | Start: 14 |
| Tm | 59.83 | 60.05 | End: 163 |
| Size | 22 | 25 | 150 |
| Primer 2 details |
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| Sequence | TCAACAAAGGTTCGTCTGTCCT | GGTTGGTACCGAATGCATATAAGT | Start: 14 |
| Tm | 59.83 | 58.939 | End: 163 |
| Size | 22 | 24 | 150 |
| Primer 3 details |
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| Sequence | TCAACAAAGGTTCGTCTGTCC | GGTTGGTACCGAATGCATATAAGTG | Start: 14 |
| Tm | 58.443 | 60.05 | End: 163 |
| Size | 21 | 25 | 150 |
| Primer 4 details |
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| Sequence | CAACAAAGGTTCGTCTGTCCT | GGTTGGTACCGAATGCATATAAGTG | Start: 15 |
| Tm | 58.438 | 60.05 | End: 163 |
| Size | 21 | 25 | 149 |
| Primer 5 details |
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| Sequence | TCAACAAAGGTTCGTCTGTCC | GGTTGGTACCGAATGCATATAAGT | Start: 14 |
| Tm | 58.443 | 58.939 | End: 163 |
| Size | 21 | 24 | 150 |
