ssr_primer
Primer Details for TRINITY_DN14458_c5_g2_i1 |
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| (T)11ggggatttatttatttatgggaatttttttcc(T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCACTCTTATCGTCGGCGG | CCCACTCCGTGATTGATGCT | Start: 158 |
| Tm | 59.9 | 60.108 | End: 282 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
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| Sequence | TTCACTCTTATCGTCGGCGG | GATCATGTTGGCAGACGCTG | Start: 158 |
| Tm | 59.9 | 59.624 | End: 305 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
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| Sequence | GGGTCCACAGTGACACACAT | GATCATGTTGGCAGACGCTG | Start: 200 |
| Tm | 59.891 | 59.624 | End: 305 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
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| Sequence | TTGGATGAAGGAGGGGTCCA | GATCATGTTGGCAGACGCTG | Start: 187 |
| Tm | 60.179 | 59.624 | End: 305 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
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| Sequence | ATGAAGGAGGGGTCCACAGT | GATCATGTTGGCAGACGCTG | Start: 191 |
| Tm | 60.179 | 59.624 | End: 305 |
| Size | 20 | 20 | 115 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCACTCTTATCGTCGGCGG | CCCACTCCGTGATTGATGCT | Start: 158 |
| Tm | 59.9 | 60.108 | End: 282 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | TTCACTCTTATCGTCGGCGG | GATCATGTTGGCAGACGCTG | Start: 158 |
| Tm | 59.9 | 59.624 | End: 305 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | GGGTCCACAGTGACACACAT | GATCATGTTGGCAGACGCTG | Start: 200 |
| Tm | 59.891 | 59.624 | End: 305 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | TTGGATGAAGGAGGGGTCCA | GATCATGTTGGCAGACGCTG | Start: 187 |
| Tm | 60.179 | 59.624 | End: 305 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | ATGAAGGAGGGGTCCACAGT | GATCATGTTGGCAGACGCTG | Start: 191 |
| Tm | 60.179 | 59.624 | End: 305 |
| Size | 20 | 20 | 115 |
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