ssr_primer
Primer Details for TRINITY_DN14442_c2_g11_i1 |
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| (T)11cctcggactgcaaacgatacataaaaattgcg(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCTCGGACTGCAAACGA | CTGAAGCTGGGAGTGACGAG | Start: 354 |
| Tm | 59.895 | 60.109 | End: 461 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
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| Sequence | ATCCTCCTCCTCGCTGTCAT | GCACTTCCTGCTCCTGTGAA | Start: 23 |
| Tm | 60.106 | 60.25 | End: 141 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
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| Sequence | TTCCTCGGACTGCAAACGAT | CTGAAGCTGGGAGTGACGAG | Start: 355 |
| Tm | 59.68 | 60.109 | End: 461 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
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| Sequence | CAACATCCTCCTCCTCGCTG | GCACTTCCTGCTCCTGTGAA | Start: 19 |
| Tm | 60.179 | 60.25 | End: 141 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
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| Sequence | TACAGTCGTGCTAGGGGAGT | TCGTTTGCAGTCCGAGGAAA | Start: 238 |
| Tm | 59.669 | 59.895 | End: 373 |
| Size | 20 | 20 | 136 |
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