ssr_primer
Primer Details for TRINITY_DN14431_c0_g3_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGGACAACAGAGTCGGAG | AATTTCTTCGCCCGGCTACA | Start: 510 |
| Tm | 60.038 | 60.036 | End: 780 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
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| Sequence | ACGGCTCCAACACAAACTCA | TCCGAACTTTCCTCTGCACC | Start: 709 |
| Tm | 60.107 | 59.965 | End: 833 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
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| Sequence | GGTGCAGAGGAAAGTTCGGA | CTTGGGGAGTGGCTTCTACG | Start: 814 |
| Tm | 59.965 | 60.108 | End: 1018 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
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| Sequence | TTCTCACGAATCCACGGCTC | TCCGAACTTTCCTCTGCACC | Start: 696 |
| Tm | 60.109 | 59.965 | End: 833 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
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| Sequence | ACGGCTCCAACACAAACTCA | ACCGTCGGCAATTTTCTCCT | Start: 709 |
| Tm | 60.107 | 59.964 | End: 816 |
| Size | 20 | 20 | 108 |
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