ssr_primer
Primer Details for TRINITY_DN14402_c1_g1_i1 |
|---|
| (G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGGGCAATCAGAAGACC | GAGCTCCTTCCGTGGTTCAA | Start: 120 |
| Tm | 59.96 | 59.965 | End: 233 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | GGGGGAAGAGTGCAGACAAA | GAGCTCCTTCCGTGGTTCAA | Start: 5 |
| Tm | 59.89 | 59.965 | End: 233 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | TTGGGGGCAATCAGAAGACC | TAGTTTGCGAGCTCCTTCCG | Start: 120 |
| Tm | 59.96 | 60.109 | End: 241 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | GGGGGAAGAGTGCAGACAAA | GGTCTTCTGATTGCCCCCAA | Start: 5 |
| Tm | 59.89 | 59.96 | End: 139 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CACCAACCCAGTCACTTCCA | GAGCTCCTTCCGTGGTTCAA | Start: 86 |
| Tm | 59.817 | 59.965 | End: 233 |
| Size | 20 | 20 | 148 |
(AGA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGGGCAATCAGAAGACC | GAGCTCCTTCCGTGGTTCAA | Start: 120 |
| Tm | 59.96 | 59.965 | End: 233 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | GGGGGAAGAGTGCAGACAAA | GAGCTCCTTCCGTGGTTCAA | Start: 5 |
| Tm | 59.89 | 59.965 | End: 233 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | TTGGGGGCAATCAGAAGACC | TAGTTTGCGAGCTCCTTCCG | Start: 120 |
| Tm | 59.96 | 60.109 | End: 241 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | GGGGGAAGAGTGCAGACAAA | GGTCTTCTGATTGCCCCCAA | Start: 5 |
| Tm | 59.89 | 59.96 | End: 139 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CACCAACCCAGTCACTTCCA | GAGCTCCTTCCGTGGTTCAA | Start: 86 |
| Tm | 59.817 | 59.965 | End: 233 |
| Size | 20 | 20 | 148 |
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