ssr_primer
Primer Details for TRINITY_DN14367_c0_g3_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCTACCAGGTTCAGGCA | GGGGGTGTGGACTAAGTGTC | Start: 118 |
| Tm | 59.884 | 59.676 | End: 355 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
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| Sequence | GGAGCCTAACGAACAAACCG | GAAGCCAGCGTGTTGGTTTT | Start: 1085 |
| Tm | 59.206 | 59.898 | End: 1213 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
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| Sequence | GGAGCCTAACGAACAAACCG | AAGCCAGCGTGTTGGTTTTG | Start: 1085 |
| Tm | 59.206 | 60.179 | End: 1212 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
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| Sequence | GGAGCCTAACGAACAAACCG | GCCAGCGTGTTGGTTTTGAA | Start: 1085 |
| Tm | 59.206 | 60.179 | End: 1210 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
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| Sequence | TTGCTGCATTGTCGTGAACA | GAAGCCAGCGTGTTGGTTTT | Start: 1004 |
| Tm | 58.98 | 59.898 | End: 1213 |
| Size | 20 | 20 | 210 |
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