Primer Details for TRINITY_DN14364_c3_g3_i1 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGAAAATATAGAACACACATCTGGT | CCTCAATTCGCTTTTGAGCCA | Start: 87 |
| Tm | 57.534 | 59.459 | End: 186 |
| Size | 27 | 21 | 100 |
| Primer 2 details |
|---|
| Sequence | AGTATCAGAGACATTGGAATCTATGCA | CCGAATGCCAGATATCTCTTGA | Start: 127 |
| Tm | 59.984 | 57.737 | End: 227 |
| Size | 27 | 22 | 101 |
| Primer 3 details |
|---|
| Sequence | AATGAAAATATAGAACACACATCTGGT | TCCTCAATTCGCTTTTGAGCC | Start: 87 |
| Tm | 57.534 | 59.187 | End: 187 |
| Size | 27 | 21 | 101 |
| Primer 4 details |
|---|
| Sequence | GTATCAGAGACATTGGAATCTATGCA | CCGAATGCCAGATATCTCTTGA | Start: 128 |
| Tm | 58.843 | 57.737 | End: 227 |
| Size | 26 | 22 | 100 |
| Primer 5 details |
|---|
| Sequence | GTATCAGAGACATTGGAATCTATGCAG | CCGAATGCCAGATATCTCTTGA | Start: 128 |
| Tm | 59.669 | 57.737 | End: 227 |
| Size | 27 | 22 | 100 |
