ssr_primer
Primer Details for TRINITY_DN14361_c1_g2_i2 |
|---|
| (T)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCACGGAAAGCATTTTGG | CGCAGCATGTTTGTCGACTC | Start: 476 |
| Tm | 59.967 | 60.179 | End: 691 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
|---|
| Sequence | GGCACGGAAAGCATTTTGGT | CGCAGCATGTTTGTCGACTC | Start: 477 |
| Tm | 59.967 | 60.179 | End: 691 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
|---|
| Sequence | GGTTGGCACGGAAAGCATTT | CGCAGCATGTTTGTCGACTC | Start: 473 |
| Tm | 59.967 | 60.179 | End: 691 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | GCTTCATGCATGTTGTGGCT | CCAAAATGCTTTCCGTGCCA | Start: 353 |
| Tm | 59.754 | 59.967 | End: 495 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
|---|
| Sequence | GCTTCATGCATGTTGTGGCT | AAATGCTTTCCGTGCCAACC | Start: 353 |
| Tm | 59.754 | 59.967 | End: 492 |
| Size | 20 | 20 | 140 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCACGGAAAGCATTTTGG | CGCAGCATGTTTGTCGACTC | Start: 476 |
| Tm | 59.967 | 60.179 | End: 691 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
|---|
| Sequence | GGCACGGAAAGCATTTTGGT | CGCAGCATGTTTGTCGACTC | Start: 477 |
| Tm | 59.967 | 60.179 | End: 691 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
|---|
| Sequence | GGTTGGCACGGAAAGCATTT | CGCAGCATGTTTGTCGACTC | Start: 473 |
| Tm | 59.967 | 60.179 | End: 691 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | GCTTCATGCATGTTGTGGCT | CCAAAATGCTTTCCGTGCCA | Start: 353 |
| Tm | 59.754 | 59.967 | End: 495 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
|---|
| Sequence | GCTTCATGCATGTTGTGGCT | AAATGCTTTCCGTGCCAACC | Start: 353 |
| Tm | 59.754 | 59.967 | End: 492 |
| Size | 20 | 20 | 140 |
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