ssr_primer
Primer Details for TRINITY_DN14360_c2_g4_i2 |
|---|
| (CGG)6ctgatgcagaagg(AGA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCTCCATCACGAGTCGAG | CCGTCCCCGTCATCTTTTCA | Start: 3082 |
| Tm | 59.971 | 60.037 | End: 3295 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | CCCCTTTTCCCACAACTCGA | CTCGACTCGTGATGGAGCTC | Start: 2974 |
| Tm | 59.89 | 59.971 | End: 3101 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
|---|
| Sequence | GAGATCACGGTGCACAATGC | CAGGTACCCCTCCCACCTTA | Start: 2347 |
| Tm | 59.902 | 59.956 | End: 2532 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GAGATCACGGTGCACAATGC | GGTACCCCTCCCACCTTAGT | Start: 2347 |
| Tm | 59.902 | 59.956 | End: 2530 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | TTCTGAGTCGATGCGGACAC | CTCCTCGTTTTCCTCCACCC | Start: 3438 |
| Tm | 60.109 | 60.036 | End: 3649 |
| Size | 20 | 20 | 212 |
(A)11(AAG)6*: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCTCCATCACGAGTCGAG | CCGTCCCCGTCATCTTTTCA | Start: 3082 |
| Tm | 59.971 | 60.037 | End: 3295 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | CCCCTTTTCCCACAACTCGA | CTCGACTCGTGATGGAGCTC | Start: 2974 |
| Tm | 59.89 | 59.971 | End: 3101 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
|---|
| Sequence | GAGATCACGGTGCACAATGC | CAGGTACCCCTCCCACCTTA | Start: 2347 |
| Tm | 59.902 | 59.956 | End: 2532 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GAGATCACGGTGCACAATGC | GGTACCCCTCCCACCTTAGT | Start: 2347 |
| Tm | 59.902 | 59.956 | End: 2530 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | TTCTGAGTCGATGCGGACAC | CTCCTCGTTTTCCTCCACCC | Start: 3438 |
| Tm | 60.109 | 60.036 | End: 3649 |
| Size | 20 | 20 | 212 |
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