ssr_primer
Primer Details for TRINITY_DN14360_c1_g2_i1 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTTTCCTTTTTCCCGCGT | TTCTCAACAATGGCGACGGA | Start: 3092 |
| Tm | 59.968 | 59.966 | End: 3225 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | AGCAGCATCAAGGCCTTCAT | ACTGCTCGACCAAATGCTGA | Start: 495 |
| Tm | 60.033 | 59.965 | End: 658 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | TTTGCAGCTTGCTCCCCATA | ACTGCTCGACCAAATGCTGA | Start: 523 |
| Tm | 59.961 | 59.965 | End: 658 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | ACAATTTCCCCGGGAAGCAT | GGCTGGACCCCTGTTCTAAC | Start: 1835 |
| Tm | 59.959 | 60.035 | End: 2036 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | ATTTGGGAGGGATGGGGAGA | ACGCGGGAAAAAGGAAAAGC | Start: 2967 |
| Tm | 59.952 | 59.968 | End: 3111 |
| Size | 20 | 20 | 145 |
(TCT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTTTCCTTTTTCCCGCGT | TTCTCAACAATGGCGACGGA | Start: 3092 |
| Tm | 59.968 | 59.966 | End: 3225 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | AGCAGCATCAAGGCCTTCAT | ACTGCTCGACCAAATGCTGA | Start: 495 |
| Tm | 60.033 | 59.965 | End: 658 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
|---|
| Sequence | TTTGCAGCTTGCTCCCCATA | ACTGCTCGACCAAATGCTGA | Start: 523 |
| Tm | 59.961 | 59.965 | End: 658 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | ACAATTTCCCCGGGAAGCAT | GGCTGGACCCCTGTTCTAAC | Start: 1835 |
| Tm | 59.959 | 60.035 | End: 2036 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | ATTTGGGAGGGATGGGGAGA | ACGCGGGAAAAAGGAAAAGC | Start: 2967 |
| Tm | 59.952 | 59.968 | End: 3111 |
| Size | 20 | 20 | 145 |
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