ssr_primer
Primer Details for TRINITY_DN14356_c0_g5_i4 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTCCGAGTTGCCCTTCAC | GCCAACAAACGTGATGCCTT | Start: 1029 |
| Tm | 59.965 | 59.968 | End: 1175 |
| Size | 20 | 20 | 147 |
| Primer 2 details |
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| Sequence | TTCTCCGAGTTGCCCTTCAC | ATGGCCCAAGTCACCTTCAG | Start: 1029 |
| Tm | 59.965 | 59.961 | End: 1210 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
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| Sequence | AATTGGGTGTTTTGGCCAGC | GTGAAGGGCAACTCGGAGAA | Start: 879 |
| Tm | 59.892 | 59.965 | End: 1048 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
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| Sequence | AATTGGGTGTTTTGGCCAGC | AGTAAGCTGCCGCTCACTTT | Start: 879 |
| Tm | 59.892 | 59.965 | End: 1027 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
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| Sequence | AAACCTCTTGGGCCTGATGG | GCAAAGGCAAGATGTGAGGC | Start: 17 |
| Tm | 59.96 | 60.109 | End: 239 |
| Size | 20 | 20 | 223 |
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