Primer Details for TRINITY_DN14353_c8_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTACACAAAAACCCTAGTTCAGG | TGTAGTCTACTTATTTCTCCCCTTGT | Start: 68 |
| Tm | 57.297 | 59.042 | End: 172 |
| Size | 24 | 26 | 105 |
| Primer 2 details |
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| Sequence | ACGAAGAACAAGTATAAGTTTTACACA | TTCTCCCCTTGTATCATTTGCT | Start: 49 |
| Tm | 57.683 | 57.409 | End: 158 |
| Size | 27 | 22 | 110 |
| Primer 3 details |
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| Sequence | TTTTACACAAAAACCCTAGTTCAGG | TGTAGTCTACTTATTTCTCCCCTTGT | Start: 67 |
| Tm | 57.897 | 59.042 | End: 172 |
| Size | 25 | 26 | 106 |
| Primer 4 details |
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| Sequence | TTTACACAAAAACCCTAGTTCAGG | TGTAGTCTACTTATTTCTCCCCTTG | Start: 68 |
| Tm | 57.297 | 57.58 | End: 172 |
| Size | 24 | 25 | 105 |
| Primer 5 details |
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| Sequence | TTTACACAAAAACCCTAGTTCAGG | GTAGTCTACTTATTTCTCCCCTTGT | Start: 68 |
| Tm | 57.297 | 57.579 | End: 171 |
| Size | 24 | 25 | 104 |
