ssr_primer
Primer Details for TRINITY_DN14339_c1_g1_i7 |
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| (T)13cttctaattattgaaattccattataccctttattcaatttgttactttctgtgctcttgtagcccctaatcacccaaatg(T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCGGGCTAAGGAAGGATC | GCCCACACCAGGATCTTGAA | Start: 1628 |
| Tm | 59.966 | 59.962 | End: 1729 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
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| Sequence | GCTCGGGCTAAGGAAGGATC | ATGCTCGTCTTCGTCAGCAA | Start: 1628 |
| Tm | 59.966 | 60.038 | End: 1813 |
| Size | 20 | 20 | 186 |
| Primer 3 details |
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| Sequence | TTCAAGATCCTGGTGTGGGC | ATGCTCGTCTTCGTCAGCAA | Start: 1710 |
| Tm | 59.962 | 60.038 | End: 1813 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
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| Sequence | AGGCTAGGTGTGAGGAGGAG | AGCTACAACCTTTCGCACCA | Start: 3109 |
| Tm | 60.033 | 59.892 | End: 3319 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
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| Sequence | GGCTAGGTGTGAGGAGGAGA | AGCTACAACCTTTCGCACCA | Start: 3110 |
| Tm | 60.033 | 59.892 | End: 3319 |
| Size | 20 | 20 | 210 |
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