Primer Details for TRINITY_DN14338_c1_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAATTGTTGCTACTCTTTCGTCT | TGTTTCTTTCCTTTTATTGAAGACCA | Start: 84 |
| Tm | 59.008 | 57.7 | End: 207 |
| Size | 25 | 26 | 124 |
| Primer 2 details |
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| Sequence | GAAATTGTTGCTACTCTTTCGTCT | TGTTTCTTTCCTTTTATTGAAGACCA | Start: 85 |
| Tm | 57.77 | 57.7 | End: 207 |
| Size | 24 | 26 | 123 |
| Primer 3 details |
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| Sequence | AGAAATTGTTGCTACTCTTTCGTC | TGTTTCTTTCCTTTTATTGAAGACCA | Start: 84 |
| Tm | 57.77 | 57.7 | End: 207 |
| Size | 24 | 26 | 124 |
| Primer 4 details |
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| Sequence | AGAAATTGTTGCTACTCTTTCGTCT | TGTTTCTTTCCTTTTATTGAAGACCAA | Start: 84 |
| Tm | 59.008 | 58.246 | End: 207 |
| Size | 25 | 27 | 124 |
| Primer 5 details |
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| Sequence | AGAAATTGTTGCTACTCTTTCGTCT | TTGTTTCTTTCCTTTTATTGAAGACCA | Start: 84 |
| Tm | 59.008 | 58.246 | End: 208 |
| Size | 25 | 27 | 125 |
