ssr_primer
Primer Details for TRINITY_DN14336_c3_g4_i8 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAAGGCTTGAGAAGAAGGG | AGGCATCACAGCAACACTCA | Start: 758 |
| Tm | 59.648 | 59.891 | End: 1023 |
| Size | 21 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | AGAGCTTGGAAGGCTTGAGA | AGGCATCACAGCAACACTCA | Start: 752 |
| Tm | 58.644 | 59.891 | End: 1023 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | AAAGACAGCACAGGCAGACA | CCCTTCTTCTCAAGCCTTCCA | Start: 531 |
| Tm | 59.819 | 59.648 | End: 778 |
| Size | 20 | 21 | 248 |
| Primer 4 details |
|---|
| Sequence | AAAGACAGCACAGGCAGACA | TCTCAAGCCTTCCAAGCTCT | Start: 531 |
| Tm | 59.819 | 58.644 | End: 771 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | AAGACAGCACAGGCAGACAG | CCCTTCTTCTCAAGCCTTCCA | Start: 532 |
| Tm | 60.25 | 59.648 | End: 778 |
| Size | 20 | 21 | 247 |
(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAAGGCTTGAGAAGAAGGG | AGGCATCACAGCAACACTCA | Start: 758 |
| Tm | 59.648 | 59.891 | End: 1023 |
| Size | 21 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | AGAGCTTGGAAGGCTTGAGA | AGGCATCACAGCAACACTCA | Start: 752 |
| Tm | 58.644 | 59.891 | End: 1023 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | AAAGACAGCACAGGCAGACA | CCCTTCTTCTCAAGCCTTCCA | Start: 531 |
| Tm | 59.819 | 59.648 | End: 778 |
| Size | 20 | 21 | 248 |
| Primer 4 details |
|---|
| Sequence | AAAGACAGCACAGGCAGACA | TCTCAAGCCTTCCAAGCTCT | Start: 531 |
| Tm | 59.819 | 58.644 | End: 771 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | AAGACAGCACAGGCAGACAG | CCCTTCTTCTCAAGCCTTCCA | Start: 532 |
| Tm | 60.25 | 59.648 | End: 778 |
| Size | 20 | 21 | 247 |
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