Primer Details for TRINITY_DN14326_c1_g3_i1 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGAAGCTTGTCTCATTTTCAAAAA | CAGGAAAGGAAACACACGTTGT | Start: 47 |
| Tm | 57.224 | 59.578 | End: 166 |
| Size | 26 | 22 | 120 |
| Primer 2 details |
|---|
| Sequence | AAAGAAGCTTGTCTCATTTTCAAAAA | TCAGGAAAGGAAACACACGTTG | Start: 47 |
| Tm | 57.224 | 59.319 | End: 167 |
| Size | 26 | 22 | 121 |
| Primer 3 details |
|---|
| Sequence | AAAGAAGCTTGTCTCATTTTCAAAAA | TCAGGAAAGGAAACACACGT | Start: 47 |
| Tm | 57.224 | 57.297 | End: 167 |
| Size | 26 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | AAAGAAGCTTGTCTCATTTTCAAAAA | AGGAAAGGAAACACACGTTGT | Start: 47 |
| Tm | 57.224 | 58.281 | End: 165 |
| Size | 26 | 21 | 119 |
| Primer 5 details |
|---|
| Sequence | AAAGAAGCTTGTCTCATTTTCAAAAA | TCAGGAAAGGAAACACACGTT | Start: 47 |
| Tm | 57.224 | 58.006 | End: 167 |
| Size | 26 | 21 | 121 |
