ssr_primer
Primer Details for TRINITY_DN14314_c2_g1_i12 |
|---|
| (A)16ggtagaacaaattccctccatatgattggacgccatcaaagctgatccttgcctttcccaccagcccaacggacacactcact(CA)9tacccacacacccccaaaacgctgacggaaccaaattctctctctctaacgcacattcccattta(CT)9: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGACAACGGTTGCTGGTTG | CCAGCCCAACCAATCCATCT | Start: 4171 |
| Tm | 59.971 | 60.033 | End: 4323 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | CAGACAACGGTTGCTGGTTG | GGTACGTTTACACCAGCCCA | Start: 4171 |
| Tm | 59.971 | 59.965 | End: 4335 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | CACCACCCGTAGCTTTCAGT | TCTGATCGGGATGGAAGGGT | Start: 4501 |
| Tm | 59.965 | 60.031 | End: 4734 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | CTGGAAAGGCTCTTGCGAGA | CAACCAGCAACCGTTGTCTG | Start: 3956 |
| Tm | 60.037 | 59.971 | End: 4190 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | TCAGTCTCAGTTTGCCGCAT | TCTGATCGGGATGGAAGGGT | Start: 4516 |
| Tm | 59.965 | 60.031 | End: 4734 |
| Size | 20 | 20 | 219 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGACAACGGTTGCTGGTTG | CCAGCCCAACCAATCCATCT | Start: 4171 |
| Tm | 59.971 | 60.033 | End: 4323 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | CAGACAACGGTTGCTGGTTG | GGTACGTTTACACCAGCCCA | Start: 4171 |
| Tm | 59.971 | 59.965 | End: 4335 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | CACCACCCGTAGCTTTCAGT | TCTGATCGGGATGGAAGGGT | Start: 4501 |
| Tm | 59.965 | 60.031 | End: 4734 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | CTGGAAAGGCTCTTGCGAGA | CAACCAGCAACCGTTGTCTG | Start: 3956 |
| Tm | 60.037 | 59.971 | End: 4190 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | TCAGTCTCAGTTTGCCGCAT | TCTGATCGGGATGGAAGGGT | Start: 4516 |
| Tm | 59.965 | 60.031 | End: 4734 |
| Size | 20 | 20 | 219 |
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