Primer Details for TRINITY_DN14310_c4_g4_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCATAGCATTGTTCAGTCGA | AGTTGGGCTTCTCTTGTAATTTTT | Start: 98 |
| Tm | 57.749 | 57.269 | End: 201 |
| Size | 21 | 24 | 104 |
| Primer 2 details | |||
| Sequence | AGCATAGCATTGTTCAGTCGAT | AGTTGGGCTTCTCTTGTAATTTTT | Start: 98 |
| Tm | 58.198 | 57.269 | End: 201 |
| Size | 22 | 24 | 104 |
| Primer 3 details | |||
| Sequence | AGCATTGTTCAGTCGATTCCA | TAGTTGGGCTTCTCTTGTAATTTTT | Start: 103 |
| Tm | 57.942 | 57.167 | End: 202 |
| Size | 21 | 25 | 100 |
| Primer 4 details | |||
| Sequence | AGCATAGCATTGTTCAGTCGATT | AGTTGGGCTTCTCTTGTAATTTTT | Start: 98 |
| Tm | 58.805 | 57.269 | End: 201 |
| Size | 23 | 24 | 104 |
| Primer 5 details | |||
| Sequence | TAGCATTGTTCAGTCGATTCCA | AGTTGGGCTTCTCTTGTAATTTTT | Start: 102 |
| Tm | 57.799 | 57.269 | End: 201 |
| Size | 22 | 24 | 100 |
