ssr_primer
Primer Details for TRINITY_DN14305_c1_g1_i1 |
|---|
| (TCA)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCTTGGAGAATCACAGGC | GGTGCAGCTGGACTTCTCTT | Start: 624 |
| Tm | 60.035 | 59.964 | End: 865 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | CACAGGGCTGCCAGGAAATA | AGAAGGCTGTGTTACGGACG | Start: 2524 |
| Tm | 60.034 | 60.039 | End: 2689 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | GTGCTTTCCCATCCACTCCA | GGTGCAGCTGGACTTCTCTT | Start: 682 |
| Tm | 59.962 | 59.964 | End: 865 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | AAGAGAAGTCCAGCTGCACC | GATCACCGAGACCGAATGCT | Start: 846 |
| Tm | 59.964 | 59.897 | End: 1082 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | CACAGGGCTGCCAGGAAATA | AAGGCTGTGTTACGGACGTT | Start: 2524 |
| Tm | 60.034 | 59.895 | End: 2687 |
| Size | 20 | 20 | 164 |
(A)11ctaagtgggaagtttgaagaagaagaaaaagaagaaaaaa(ATG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCTTGGAGAATCACAGGC | GGTGCAGCTGGACTTCTCTT | Start: 624 |
| Tm | 60.035 | 59.964 | End: 865 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | CACAGGGCTGCCAGGAAATA | AGAAGGCTGTGTTACGGACG | Start: 2524 |
| Tm | 60.034 | 60.039 | End: 2689 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | GTGCTTTCCCATCCACTCCA | GGTGCAGCTGGACTTCTCTT | Start: 682 |
| Tm | 59.962 | 59.964 | End: 865 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | AAGAGAAGTCCAGCTGCACC | GATCACCGAGACCGAATGCT | Start: 846 |
| Tm | 59.964 | 59.897 | End: 1082 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | CACAGGGCTGCCAGGAAATA | AAGGCTGTGTTACGGACGTT | Start: 2524 |
| Tm | 60.034 | 59.895 | End: 2687 |
| Size | 20 | 20 | 164 |
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