ssr_primer
Primer Details for TRINITY_DN14300_c1_g6_i2 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGCCCTAACGCCAAAACC | TTACGCGTTCTCCCTCAACC | Start: 713 |
| Tm | 60.039 | 60.038 | End: 990 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | GGTTGAGGGAGAACGCGTAA | TTGCTTCTTTTGGAGGCCCA | Start: 971 |
| Tm | 60.038 | 60.106 | End: 1169 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | TGAGGGAGAACGCGTAACAC | TTGCTTCTTTTGGAGGCCCA | Start: 974 |
| Tm | 60.039 | 60.106 | End: 1169 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | CAAAGCGGACCAACTTCACG | TTGCTTCTTTTGGAGGCCCA | Start: 1035 |
| Tm | 60.041 | 60.106 | End: 1169 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GGTTGAGGGAGAACGCGTAA | AAGGTGGCCTTTGATGACGT | Start: 971 |
| Tm | 60.038 | 59.89 | End: 1116 |
| Size | 20 | 20 | 146 |
(AT)6acaataaagcaatttatatag(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGCCCTAACGCCAAAACC | TTACGCGTTCTCCCTCAACC | Start: 713 |
| Tm | 60.039 | 60.038 | End: 990 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | GGTTGAGGGAGAACGCGTAA | TTGCTTCTTTTGGAGGCCCA | Start: 971 |
| Tm | 60.038 | 60.106 | End: 1169 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | TGAGGGAGAACGCGTAACAC | TTGCTTCTTTTGGAGGCCCA | Start: 974 |
| Tm | 60.039 | 60.106 | End: 1169 |
| Size | 20 | 20 | 196 |
| Primer 4 details |
|---|
| Sequence | CAAAGCGGACCAACTTCACG | TTGCTTCTTTTGGAGGCCCA | Start: 1035 |
| Tm | 60.041 | 60.106 | End: 1169 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GGTTGAGGGAGAACGCGTAA | AAGGTGGCCTTTGATGACGT | Start: 971 |
| Tm | 60.038 | 59.89 | End: 1116 |
| Size | 20 | 20 | 146 |
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