ssr_primer
Primer Details for TRINITY_DN14300_c1_g1_i12 |
|---|
| (GT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAGTCTTTCCCTCTGGGC | CAAGTTTTCGGCGTCACGTT | Start: 1645 |
| Tm | 60.035 | 59.973 | End: 1818 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | CGTTCTCACTGGCTGGAGTT | GTGTTGTGCCGTTGGAACTC | Start: 1096 |
| Tm | 59.966 | 59.971 | End: 1248 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | TCACTTCTGGTTCCTTCGCC | GTGTTGTGCCGTTGGAACTC | Start: 1074 |
| Tm | 59.965 | 59.971 | End: 1248 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | GCAACCATCACGTTTCCACC | CAAGTTTTCGGCGTCACGTT | Start: 1580 |
| Tm | 60.04 | 59.973 | End: 1818 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | CTCCCAAATCGGCTTCCTGT | TGACGAGTCTGATGGGCCTA | Start: 371 |
| Tm | 60.035 | 60.034 | End: 564 |
| Size | 20 | 20 | 194 |
(TTC)5aatttctcttaaaaaagaaccaat(ACA)7(A)12*: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAGTCTTTCCCTCTGGGC | CAAGTTTTCGGCGTCACGTT | Start: 1645 |
| Tm | 60.035 | 59.973 | End: 1818 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | CGTTCTCACTGGCTGGAGTT | GTGTTGTGCCGTTGGAACTC | Start: 1096 |
| Tm | 59.966 | 59.971 | End: 1248 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | TCACTTCTGGTTCCTTCGCC | GTGTTGTGCCGTTGGAACTC | Start: 1074 |
| Tm | 59.965 | 59.971 | End: 1248 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | GCAACCATCACGTTTCCACC | CAAGTTTTCGGCGTCACGTT | Start: 1580 |
| Tm | 60.04 | 59.973 | End: 1818 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | CTCCCAAATCGGCTTCCTGT | TGACGAGTCTGATGGGCCTA | Start: 371 |
| Tm | 60.035 | 60.034 | End: 564 |
| Size | 20 | 20 | 194 |
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