Primer Details for TRINITY_DN14295_c2_g1_i1 | |||
|---|---|---|---|
| (AT)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGCTCAGAAGTACCAAGTGA | ACTATAGTGAGTTTGCACCGT | Start: 90 |
| Tm | 59.034 | 57.044 | End: 190 |
| Size | 22 | 21 | 101 |
| Primer 2 details | |||
| Sequence | AGTGCTCAGAAGTACCAAGTGA | ACTATAGTGAGTTTGCACCGTT | Start: 90 |
| Tm | 59.034 | 57.733 | End: 190 |
| Size | 22 | 22 | 101 |
| Primer 3 details | |||
| Sequence | ACCACATCAATGAAATGGTTTGTGT | AGTGAGTTTGCACCGTTTTGA | Start: 26 |
| Tm | 60.163 | 58.912 | End: 185 |
| Size | 25 | 21 | 160 |
| Primer 4 details | |||
| Sequence | TGCACATAGAAATGGTGCTTCC | TCATGGTTACTTAGCCAAGAGTGA | Start: 124 |
| Tm | 59.245 | 59.469 | End: 233 |
| Size | 22 | 24 | 110 |
| Primer 5 details | |||
| Sequence | GTGCTCAGAAGTACCAAGTGA | ACTATAGTGAGTTTGCACCGT | Start: 91 |
| Tm | 57.613 | 57.044 | End: 190 |
| Size | 21 | 21 | 100 |
