Primer Details for TRINITY_DN14294_c2_g1_i1 |
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| (T)13(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACATCGTTATAATGCTTTTCAGTCA | CTGACACACTCACTGACGCT | Start: 6 |
| Tm | 59.881 | 59.969 | End: 152 |
| Size | 27 | 20 | 147 |
| Primer 2 details |
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| Sequence | ACACATCGTTATAATGCTTTTCAGTCA | ATGCCCTGACACACTCACTG | Start: 6 |
| Tm | 59.881 | 59.964 | End: 157 |
| Size | 27 | 20 | 152 |
| Primer 3 details |
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| Sequence | ACACATCGTTATAATGCTTTTCAGT | CTGACACACTCACTGACGCT | Start: 6 |
| Tm | 57.622 | 59.969 | End: 152 |
| Size | 25 | 20 | 147 |
| Primer 4 details |
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| Sequence | ACACATCGTTATAATGCTTTTCAGT | ATGCCCTGACACACTCACTG | Start: 6 |
| Tm | 57.622 | 59.964 | End: 157 |
| Size | 25 | 20 | 152 |
| Primer 5 details |
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| Sequence | ACACATCGTTATAATGCTTTTCAGTCA | CCCTGACACACTCACTGACG | Start: 6 |
| Tm | 59.881 | 60.319 | End: 154 |
| Size | 27 | 20 | 149 |
(T)28: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACATCGTTATAATGCTTTTCAGTCA | CTGACACACTCACTGACGCT | Start: 6 |
| Tm | 59.881 | 59.969 | End: 152 |
| Size | 27 | 20 | 147 |
| Primer 2 details |
|---|
| Sequence | ACACATCGTTATAATGCTTTTCAGTCA | ATGCCCTGACACACTCACTG | Start: 6 |
| Tm | 59.881 | 59.964 | End: 157 |
| Size | 27 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | ACACATCGTTATAATGCTTTTCAGT | CTGACACACTCACTGACGCT | Start: 6 |
| Tm | 57.622 | 59.969 | End: 152 |
| Size | 25 | 20 | 147 |
| Primer 4 details |
|---|
| Sequence | ACACATCGTTATAATGCTTTTCAGT | ATGCCCTGACACACTCACTG | Start: 6 |
| Tm | 57.622 | 59.964 | End: 157 |
| Size | 25 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | ACACATCGTTATAATGCTTTTCAGTCA | CCCTGACACACTCACTGACG | Start: 6 |
| Tm | 59.881 | 60.319 | End: 154 |
| Size | 27 | 20 | 149 |
