ssr_primer
Primer Details for TRINITY_DN14281_c3_g4_i1 |
|---|
| (AT)9aactcccaaatatattatatataac(AAG)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACCAGTCCAATGCTCAT | AGTTGCTTGACATCCAGCGA | Start: 415 |
| Tm | 59.961 | 59.965 | End: 584 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | AACCCCCTGACTGCTCAATG | Start: 415 |
| Tm | 59.961 | 59.961 | End: 607 |
| Size | 20 | 20 | 193 |
| Primer 3 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | CAACCCCCTGACTGCTCAAT | Start: 415 |
| Tm | 59.961 | 59.961 | End: 608 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | GGGGCTCCAAGGATCAACAA | Start: 415 |
| Tm | 59.961 | 59.96 | End: 562 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
|---|
| Sequence | CTTGTCAGCCCCTCCCAATT | ATGGGGGTGGAGGAGATTCA | Start: 1787 |
| Tm | 59.96 | 59.954 | End: 1908 |
| Size | 20 | 20 | 122 |
(AT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACCAGTCCAATGCTCAT | AGTTGCTTGACATCCAGCGA | Start: 415 |
| Tm | 59.961 | 59.965 | End: 584 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | AACCCCCTGACTGCTCAATG | Start: 415 |
| Tm | 59.961 | 59.961 | End: 607 |
| Size | 20 | 20 | 193 |
| Primer 3 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | CAACCCCCTGACTGCTCAAT | Start: 415 |
| Tm | 59.961 | 59.961 | End: 608 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | GGGGCTCCAAGGATCAACAA | Start: 415 |
| Tm | 59.961 | 59.96 | End: 562 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
|---|
| Sequence | CTTGTCAGCCCCTCCCAATT | ATGGGGGTGGAGGAGATTCA | Start: 1787 |
| Tm | 59.96 | 59.954 | End: 1908 |
| Size | 20 | 20 | 122 |
(GCT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACCAGTCCAATGCTCAT | AGTTGCTTGACATCCAGCGA | Start: 415 |
| Tm | 59.961 | 59.965 | End: 584 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | AACCCCCTGACTGCTCAATG | Start: 415 |
| Tm | 59.961 | 59.961 | End: 607 |
| Size | 20 | 20 | 193 |
| Primer 3 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | CAACCCCCTGACTGCTCAAT | Start: 415 |
| Tm | 59.961 | 59.961 | End: 608 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | GGGGCTCCAAGGATCAACAA | Start: 415 |
| Tm | 59.961 | 59.96 | End: 562 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
|---|
| Sequence | CTTGTCAGCCCCTCCCAATT | ATGGGGGTGGAGGAGATTCA | Start: 1787 |
| Tm | 59.96 | 59.954 | End: 1908 |
| Size | 20 | 20 | 122 |
(GAA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACCAGTCCAATGCTCAT | AGTTGCTTGACATCCAGCGA | Start: 415 |
| Tm | 59.961 | 59.965 | End: 584 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | AACCCCCTGACTGCTCAATG | Start: 415 |
| Tm | 59.961 | 59.961 | End: 607 |
| Size | 20 | 20 | 193 |
| Primer 3 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | CAACCCCCTGACTGCTCAAT | Start: 415 |
| Tm | 59.961 | 59.961 | End: 608 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
|---|
| Sequence | TGCACCAGTCCAATGCTCAT | GGGGCTCCAAGGATCAACAA | Start: 415 |
| Tm | 59.961 | 59.96 | End: 562 |
| Size | 20 | 20 | 148 |
| Primer 5 details |
|---|
| Sequence | CTTGTCAGCCCCTCCCAATT | ATGGGGGTGGAGGAGATTCA | Start: 1787 |
| Tm | 59.96 | 59.954 | End: 1908 |
| Size | 20 | 20 | 122 |
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