ssr_primer
Primer Details for TRINITY_DN14270_c1_g4_i1 |
|---|
| (AG)6atgtaaagg(A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACCCTCTCCCTCATCCAC | CCATGAGACAGAGCCTGCAA | Start: 471 |
| Tm | 60.032 | 60.036 | End: 736 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TTGCAGGCTCTGTCTCATGG | AGGACGCATCGAATGGTTGT | Start: 717 |
| Tm | 60.036 | 60.036 | End: 968 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | TTGCAGGCTCTGTCTCATGG | GCCTCTGTTTTCTTGCAGGC | Start: 717 |
| Tm | 60.036 | 60.039 | End: 873 |
| Size | 20 | 20 | 157 |
| Primer 4 details |
|---|
| Sequence | GCCTGCAAGAAAACAGAGGC | AGGACGCATCGAATGGTTGT | Start: 854 |
| Tm | 60.039 | 60.036 | End: 968 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | TTGCAGGCTCTGTCTCATGG | CCACGCCTCTGTTTTCTTGC | Start: 717 |
| Tm | 60.036 | 60.04 | End: 877 |
| Size | 20 | 20 | 161 |
(A)10(AAT)5*tctttcaccaagtattaagttaatggtgatagttgtacatggaagaagatgat(GAA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACCCTCTCCCTCATCCAC | CCATGAGACAGAGCCTGCAA | Start: 471 |
| Tm | 60.032 | 60.036 | End: 736 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TTGCAGGCTCTGTCTCATGG | AGGACGCATCGAATGGTTGT | Start: 717 |
| Tm | 60.036 | 60.036 | End: 968 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | TTGCAGGCTCTGTCTCATGG | GCCTCTGTTTTCTTGCAGGC | Start: 717 |
| Tm | 60.036 | 60.039 | End: 873 |
| Size | 20 | 20 | 157 |
| Primer 4 details |
|---|
| Sequence | GCCTGCAAGAAAACAGAGGC | AGGACGCATCGAATGGTTGT | Start: 854 |
| Tm | 60.039 | 60.036 | End: 968 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | TTGCAGGCTCTGTCTCATGG | CCACGCCTCTGTTTTCTTGC | Start: 717 |
| Tm | 60.036 | 60.04 | End: 877 |
| Size | 20 | 20 | 161 |
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